Comparative molecular evaluation of fungi degraded date pits as a dietary supplement for aflatoxicosis induced in fish and rats animal models.
Nehal Elkhouly1, Hanaa Assem1, Doaa A.Ghareeb2, Mohamed El-Sayed2Maha El
1National Institute Of Oceanography and Fisheries, Alexandria, Egypt.
2Biochemistry Department, Faculty of Science, Alexandria University, Egypt.
- City of Scientific Research And Technological Applications Borg El-Arab, Alexandria, Egypt
Corresponding E-mail: firstname.lastname@example.org
Keywords: FDDP, Aflatoxin, Aquaculture, Hepatoprotective.
Mycotoxins are produced as secondary metabolites by moulds on agricultural products before or after harvest and also during transport and storage. Among the mycotoxins, Aflatoxins, which are the most toxic and have considerable interest in agriculture, livestock and aquaculture. Chronic exposure to aflatoxin contaminated feeds lead to HCC in fishes or humans consumed foods contaminated with it or their metabolites. Egypt has the highest prevalence rate of HCC. Therefore, the found of new safe inhibitor with no toxicity is the passion of scientist work in this area. Mainly, products that derived from natural product give these desirable effects as they act as anti- oxidant and anti -inflammatory like date pits extract to reduce the free radicals liberated by aflatoxins which causing cell damage and carcinogenicity. Therefore, our objective is to introduce a novel cheap by- product fungi degraded date pits (FDDP) supplement and to estimate the potency of FDDP as a new novel dietary supplement without side effects for the aflatoxicosis prevention and/ or treatment. This done through series of in vitro and in vivo experiments in which the effect of FDDP will be examined on HepG2 cell line. Moreover, the composition of FDDP studied by using different chromatographic and spectroscopic techniques. The bioactivities of FDDP suspension will be assessed by using chemical methods. Furthermore, the same set of experiments carried out on marine and animal model (Nile Tilapia fish and Rats) to estimate the efficacy of this FDDP supplemented diets as preventive and or curative supplement against aflatoxicosis by studying biochemical and molecular markers. Therefore, the finding of a new cheap dietary supplement against aflatoxicosis will solve a great economic problem either in aquaculture (fish feeds) or in landscape where we will provide a protective dietary supplement against aflatoxin contaminated feeds. Our work is now in progress under the supervision and funding of STDF organization The results of this project will be informative to be disseminated in a very well form by: Providing an innovated therapeutic compound isolated from natural resources which gives it the priority to be used in pharmaceutical market because they lesser side effects. Therefore the main outcomes are;
- Establishment of complete in vitro and preclinical trail to prove the compound efficiency.
- Open the gate to the prototype of this compound.
- Introduce a new pharmaceutical compound for Aflatoxicosis treatment.